Tropoelastin
Human Tropoelastin, Lyophilized, 1 mg
Catalog #5052-1MG (formerly #5052)
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Directions for UseProduct BrochureSDSCertificate of OriginReferencesCertificate of Analysis |
Full Product Description
Tropoelastin is the precursor to the elastin molecule. Elastin is found in many tissues of the body including the skin, arterial walls and ligaments. Elastin confers strength and elasticity to tissues and organs.
Tropoelastin is not normally available in its native state since it becomes cross-linked immediately after its synthesis by the cell, during its export into the extracellular matrix.
This tropoelastin is produced using recombinant production methods. The product is supplied as a sterile, lyophilized powder containing 1 mg per vial. A Certificate of Analysis is available with the purchase of each product.
Tropoelastin is typically used as a thin coating on tissue culture surfaces. Specific instructions are found in the Directions for Use. This product is generally used in vitro as a substrate scaffold to enhance cell attachment, adherence and proliferation.
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Parameter, Testing, and Method |
Tropoelastin |
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Form |
Lyophiilzed Powder |
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Package Size |
1 mg |
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Sterilization Method |
Irradiation |
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Storage Temperature |
-20 °C prior to reconstitution |
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Expiration Date |
Listed on product label and |
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Shelf Life |
24 Months |
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Shelf Life after Reconstitution |
3 Months |
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Polyacrylamide Gel Electrophoresis |
Characteristic |
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Sterility |
No Growth |
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Endotoxin |
< 1.0 EU/ml |
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Cell Attachment Assay |
Pass |
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Source |
Recombinant |
Cell Attachment Bioassay
To demonstrate cell attachment, cells were seeded onto surfaces coated with the matrix product and onto negative control surfaces without the matrix product. Cells were seeded onto surfaces using serum-free DMEM media. All surfaces were blocked with a solution containing 1% BSA. Cells were then allowed to attach for one (1) hour at 37°C. The culture surfaces were washed with DPBS. The results indicate significant cell attachment with surfaces coated using the matrix product.
| Without Tropoelastin | With Tropoelastin |
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| Human Umbilical Vein Endothelial Cells (HUVEC) 10X | |
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| VERO Epithelial Cells 10X | |
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| Human Dermal Fibroblasts (10X) | |
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| MDCK Epithelial Cells 10X | |
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