Bovine Collagen Solution, Type V
Bovine Collagen Solution, Type V, 0.5 mg, ~ 0.2 mg/ml
Catalog #5168-0.5MG (formerly #5168)
Full Product Description
Type V Collagen provides structure and strength to connective tissue and is found in many places in the body, especially skin, lung, intestinal walls and walls of blood vessels. This Type V Collagen product is isolated from placenta and is purified using a multi-step process with a final purity of >95%. Type V collagen forms a triple helix composed of a Alpha 1(V)2 Alpha 2(V) heterotrimer.
The product is supplied as a sterile solution at 0.5 mg per vial at approximately 0.15 to 0.30 mg/ml in 100 mM acetic acid. A Certificate of Analysis is available with the purchase of each product. Type V collagen product is provided in a user-friendly packaging for use and storage.
Type V Collagen is typically used as a thin coating on tissue culture surfaces. Specific instructions are found in the Directions for Use. This product is generally used in vitro as a substrate scaffold to enhance cell attachment, adherence and proliferation. Type V collagen may be used to culture a variety of cell types.
|Parameter, Testing, and Method||Type V Collagen Solution
Catalog # 5168-0.5MG
|Package Size||0.5 mg|
|Formulation Buffer||100 mM Acetic Acid|
|Volume||~ 2 ml|
|Concentration||Approx. 0.15 to 0.30 mg/ml – concentration
is listed on label
|pH||Approx. pH 3|
|Storage Temperature||2-10 °C|
|Purity by SDS – PAGE Polyacrylamide Gel Electrophoresis||Characteristic Banding
|Endotoxin (LAL)||< 5.0 EU/ml|
Preparation and Usage
Note: Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.
- Remove required quantity of collagen from the bottle and dispense into a dilution vessel.
- Dilute Type V Collagen with 100 mM acetic acid to ~10 µg/ml.
- Swirl contents gently until material is completely mixed.
- Add appropriate amount of diluted Type V Collagen material to the culture surface ensuring that the entire surface is coated.
- Incubate at room temperature, covered, for 1-2 hours.
- After incubation, aspirate any remaining material.
- Rinse coated surfaces carefully with sterile medium or PBS, avoid scratching surfaces.
- Coated surfaces are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.