Human Collagen, Type III
Human Collagen Solution, Type III, 10 mg, ~ 1 mg/ml
Catalog #5021-10MG (formerly #5021)
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Directions for UseSDSCertificate of OriginCertificate of Analysis |
Full Product Description
Type III Collagen provides structure and strength to connective tissue, is found many places in the body, especially skin, lung, intestinal walls and the walls of blood vessels. Collagen III is initially produced as procollagen, a protein consisting of three pro-alpha1(III) chains that form the triple-stranded, rope-like molecule. After being synthesized, the procollagen molecule is modified by the cell. Enzymes modify the amino acids lysine and proline in the protein strands by adding chemical groups that are necessary for the strands to form a stable molecule and then later to crosslink to other molecules outside the cell. The Type III procollagen molecules are released from the cell and are processed by enzymes that clip small segments off either end of the molecules to form mature collagen. The mature collagen molecules assemble into fibrils. Cross-linking between molecules produces a very stable fibril, contributing to collagen’s tissue strengthening function.
This Type III Collagen product is isolated from human placenta and is purified using a multi-step process with approximately 85% Type III collagen with the remainder being comprised of Type I collagen. The product is supplied as a sterile solution with 10 mg at approximately 1 mg/ml in 0.01 N HCl, pH 2. A Certificate of Analysis is available with the purchase of each product. Type III collagen product is provided in a user-friendly packaging for use and storage. This Type III collagen product is sterile filtered and is supplied as a ready to use solution.
Type III Collagen is typically used as a thin coating on tissue culture surfaces or as a control. Specific instructions are found in the Directions for Use. This product is generally used in vitro as a substrate scaffold to enhance cell attachment, adherence and proliferation. Type III collagen may be used to culture a variety of cell types.
Parameter, Testing, and Method |
Type III Collagen Solution |
Form |
Solution |
Package Size |
10 mg |
Storage Temperature |
2-10 °C |
Shelf Life |
24 months |
Concentration |
Approximately 1 mg/ml – concentration |
pH |
Approx. pH 2, 0.01 N HCl |
Polyacrylamide Gel Electrophoresis |
Characteristic |
Sterility |
No growth |
Endotoxin (LAL) |
< 10.0 EU/ml |
Adventitious Agents |
The human source material has been tested and found negative for hepatitis B virus, hepatitis C virus, and for human immunodeficiency |
Source |
Placenta, Human |
Preparation and Usage
Coating Procedure
Note: Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.
- Remove required quantity of collagen from the bottle and dispense into a dilution vessel.
- Dilute Type III collagen solution with a 0.01 M HCl solution water to ~50 to 100 µg/ml (~1:10).
- Swirl contents gently until material is completely mixed.
- Add appropriate amount of diluted Type III Collagen to the culture surface ensuring that the entire surface is coated.
- Incubate at room temperature, covered for 1-2 hours.
- After incubation, aspirate any remaining material.
- Rinse coated surfaces carefully with sterile medium or PBS, avoid scratching surfaces.
- Coated surfaces are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.
3-D Gel Preparation Procedure
- Slowly add 1 part of chilled 10X PBS or 10X culture media to 8 parts of chilled collagen solution with gentle swirling.
- Adjust pH of mixture to 7.2–7.6 using sterile 0.1 M NaOH. Monitor pH adjustment carefully (pH meter, phenol red, or pH paper).
- Adjust final volume to a total of 10 parts with sterile water.
- To prevent gelation, maintain temperature of mixture at 2–10°C.
- To form gel, warm to 37°C. Allow approximately 90 to 120 minutes for gel formation.