Bovine Collagen Solution, Type II
Bovine Collagen Solution, Type II, 0.5 mg/mL, 1mL mg/ml
Full Product Description
Bovine Collagen Type II is one of a family of proteins found particularly in the flesh and connective tissues of mammals (approximately one-third of the body’s total protein). Over two dozen types of collagen have been described; Type II is found predominantly in hyaline cartilage (50% of the protein in hyaline is collagen type II) and is also found in the vitreous humor of the eye.
The product is supplied as a sterile solution at approximately 0.5 mg per vial at approximately ~.50 mg/ml in 500 mM acetic acid. A certificate of Analysis is available with the purchase of each product. Type II collagen is provided in user-friendly packaging for use and storage. Type II collagen may be used to culture a variety of cell types. Type II collagen is typically used as a coating material for cell culture studies, for the formation of 3D collagen gels, or as an antigen for antibody production.
|Parameter, Testing, and Method||Type II Collagen Solution
Catalog # 5196-0.5MG
|Package Size||0.5 mg|
|Formulation Buffer||500 mM Acetic Acid|
|Volume||~ 1 ml|
|Concentration||Approx. 0.50 mg/mL|
|pH||Approx. pH 2.5|
|Storage Temperature||2-10 °C|
|Purity by SDS – PAGE Polyacrylamide Gel Electrophoresis||Characteristic Banding
|Endotoxin (LAL)||< 5.0 EU/ml|
Preparation and Usage
Note: Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.
- Remove required quantity of collagen from the bottle and dispense into a dilution vessel.
- Dilute the product with a 500 mM acetic acid solution to desired concentration.
- Swirl contents gently until thoroughly mixed.
- Add approximate amount of diluted Type II Collagen to the culture surface ensuring the entire surface is coated.
- Incubate at room temperature, covered for 1-2 hours.
- After incubation, aspirate any excess remaining material.
- Rinse coated surface carefully with a sterile medium or balanced salt solution. Avoid scratching surfaces.
- Aspirate remaining material from coated surface.
- Coated culture vessels are now ready to use. The coated culture vessels may be stored at 2 to 10°C