FlexiCol®
FlexiCol® – Porcine Collagen Solution, Type I, 3 mg/ml, 100 ml
Catalog #5169-100ML (formerly #5169-B)
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Directions for UseProduct BrochureSDSCertificate of OriginCertificate of Analysis3D Gel Video |
Full Product Description
FlexiCol® is isolated from porcine hides and is a soluble atelo-collagen in 0.01 N HCI. FlexiCol® collagen is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. FlexiCol® collagen is supplied at approximately a 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. FlexiCol® collagen is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel. FlexiCol® collagen is provided in a user-friendly packaging for use and storage. FlexiCol® is sterile filtered and is supplied as a ready to use solution.
Parameter, Testing, and Method | FlexiCol® Collagen Solution Catalog # 5169-100ML |
Sterilization Method | Filtration |
Form | Solution |
Package Size | 100 ml |
Storage Temperature | 2-10 °C |
Expiration Date | Listed on product label and Certificate of Analysis |
Concentration (Biuret Protein Determination) |
2.9-3.3 mg/ml |
pH | Approx. pH 2, 0.01 N HCl |
Gel Time (Gel Time Assay) |
< 40 minutes |
Fibrillogenesis (Fibril Formation) |
>0.5 Absorbance Units |
Polyacrylamide Gel Electrophoresis (SDS – PAGE) |
> 85% collagen containing within alpha, beta, and gamma bands < 15% collagen contained with bands traveling faster than alpha |
Sterility | No growth |
Endotoxin (LAL) | < 1.0 EU/ml |
Osmolality | ~ 30 mOsm |
Cell Attachment Assay | Pass |
Source | Porcine Hide – Pepsin Extracted |
Preparation and Usage
Coating Procedure
Note:Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.
- Remove required quantity of collagen from the bottle and dispense into a dilution vessel.
- Dilute FlexiCol® in water to ~50 to 100 µg/ml (~1:30). A 0.01 N HCl solution may also be used.
- Swirl contents gently until material is completely mixed.
- Add appropriate amount of diluted FlexiCol® material to the culture surface ensuring that the entire surface is coated.
- Incubate at room temperature, covered, for 1-2 hours. Aspirate any remaining material. Alternatively, incubate at room temperature until surface is dry.
- Rinse coated surfaces carefully with sterile medium or PBS, avoid scratching surfaces.
- Coated surfaces are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.
3-D Gel Preparation Procedure
- Slowly add 1 part of chilled 10X PBS or 10X culture media to 8 parts of chilled collagen solution with gentle swirling.
- Adjust pH of mixture to 7.2–7.6. Use of sterile 0.1 M NaOH is recommended. Monitor pH adjustment carefully (pH meter, phenol red, or pH paper).
- Adjust final volume to a total of 10 parts with sterile water.
- To prevent gelation, maintain temperature of mixture at 2-10°C.
- To form gel, warm to 37°C. For best results allow approximately 90 to 120 minutes for gel formation.
Cell Attachment Bioassay
Without FlexiCol® | With FlexiCol® |
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Human Umbilical Vein Endothelial Cells (HUVEC) 10X | |
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VERO Epithelial Cells 10X | |
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Human Dermal Fibroblast Cells 10X | |
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MDCK Epithelial Cells 10X |