CytoForm®-100

Cell Seeding Instructions

1. Fill each well containing a scaffold with 3.5 mL of cell culture medium (approximately half the well volume) one day prior to seeding to wet the scaffold. Incubate at 37°C.
2. After incubation, aspirate off the cell culture medium.
3. Add cells the next day by dispensing on top of the scaffold and allow cells to attach for 30-60 minutes.
4. Fill the well with cell culture medium until the scaffold is completely covered.

Note: The type of medium used and exact cell numbers depend on the cell type. As a reference, we recommend ~5.0 x 10⁴  to 1.0 x 10⁵ human bone marrow stromal cells per scaffold.

Cell Seeding Instructions Using Collagen Hydrogel

1. Fill each well containing a scaffold with 3.5 mL of cell culture medium (approximately half the well volume) one day prior to seeding to wet the scaffold. Incubate at 37°C.
2. After incubation, aspirate off the cell culture medium.
3. Add cells to a neutralized collagen solution (such as PureCol^® EZ Gel).

Note: The type of medium used and exact cell numbers depend on the cell type. As a reference, we recommend ~5.0 x 10⁴  to 1.0 x 10⁵ human bone marrow stromal cells per scaffold.

4. Slowly dispense the collagen/cell solution over the scaffold and allow the solution to fully permeate throughout the scaffold.
5. Allow collagen to polymerize within the scaffold for 60-90 minutes at 37°C.
6. Fill the well with cell culture medium until the scaffold is completely covered.