Highly Concentrated Type I Collagen BioInk
Full Product Description
Three dimensional (3D) gels allow for the study of the effects of the mechanical properties of the extracellular matrix (ECM), such as density and rigidity, on cell development, migration, and morphology. Unlike 2D systems, 3D environments allow cell extensions to simultaneously interact with integrins on all cell surfaces, resulting in the activation of specific signaling pathways. Gel stiffness or rigidity also affects cell migration differently in 3D versus 2D environments.
A bioink that resembles life should include the following traits: High Print Resolution, shear-thinning, strong mechanical strength, superior cytocompatibility, allows for cellular remodeling, and is biomimetic. LifeInk® 200 fulfills those requirements.
Advanced BioMatrix offers LifeInk® 200, a bioink that is a highly concentrated type I collagen. LifeInk® 200 is pH neutral collagen with physiological salt concentration.
The LifeInk® 200 kit consists of highly concentrated collagen, as the core component with additional syringes and mixing couplers.
|Item||Catalog No.||Package Size|
|Collagen, Type I, Highly Concentrated||5199-5ML||5 mL|
|Sterile 10cc Syringe|
|Sterile Syringe Coupler|
The highly concentrated collagen is extracted from bovine hide and contains a high monomer content. The collagen starting material and purified using controlled manufacturing processes.
LifeInk® 200 bioink has the following characteristics as shown in Table 2.
|Purity by SDS PAGE electrophoresis||> 98%|
|Gel Stiffness||See graph 1 below|
|Endotoxin||< 10 Eu/ml|
Preparation and Usage
Preparation Instructions for LifeInk® 200
Note: Employ aseptic practices to maintain the sterility of the product throughout the preparation and handling of the collagen and other solutions.
Note: It is recommended that the collagen and other working solutions be chilled during the preparation of the collagen.
Note: Vortexing of collagen is not recommended at any step.
Note: Ensure that NO bubbles enter the system. Bubbles in the system while mixing will turn your ink into a foam-like material.
Cell Addition to BioInk:
Note: Keep collagen chilled throughout these processes
- Add concentrated chilled cells to a sterile 10cc syringe.
- Place sterile coupler on the end of the syringe with the collagen.
- Slowly push plunger in until collagen forms a slight external meniscus above the end of the coupler on the syringe.
- Remove cap from 10cc syringe with cell seeding solution and slowly push plunger in until cell seeding solution forms a slight external meniscus above the end of the syringe.
- Couple the syringe with cells to the syringe with collagen.
Push plungers back and forth >40 times to ensure thorough mixing (starting with the collagen syringe). End with all of the material in the 10cc collagen syringe.
Note: The cell-laden bioink is now ready.
Note: To use smaller quantities of collagen, first simply transfer desired amount of collagen from the 10cc syringe to another syringe using the provided sterile coupler.
General Printing Notes:
- Complete your print within 1 hour of cell addition to syringe/bioink.
- When printing with FRESH gelatin slurry, allow the structure to incubate at 37C for 30 minutes to an hour, and then replace the gelatin with media.
- We recommend a final cell concentration of 5 x 106 cells/mL.
- Add 2 mL of cell suspension media per 5 mL of collagen bioink.
- Avoid bubbles.
Cell Attachment Bioassay