CD2
Human, Recombinant, 0.1 mg
Catalog #5086
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Directions for UseMSDSCertificate of Analysis
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Full Product Description
Full-length extracellular domain of human CD2 gene (25 – 209 aa) was constructed with 31 N-terminal T7/His tag and expressed in E. coli as inclusion bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified.
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Parameter, Testing, and Method |
CD2, Human, Recombinant |
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Quantity |
0.1 mg (100 µg/vial) |
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Volume |
0.2 mL |
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Concentration |
0.5 mg/mL |
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Purity |
>90% |
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Formulation |
Formulated in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, L-Arginine, DTT and Glycerol. |
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Form |
Solution |
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Production Type |
Recombinant – E. coli |
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Storage Temperature |
-20 °C |
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Shelf Life |
12 months after receipt |
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Sterilization Method |
Filtration |
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Cell Attachment Activity |
Passes |
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Sterility |
No growth |
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Accession Number |
NP_001758 |
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Recombinant Protein Sequence |
MASMTGGQQMGRGHHHHHHGNLYFQGGEFEL |
Preparation and Usage
Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.
- Thaw CD2 and dilute to desired concentration using serum-free medium or PBS. The final solution should be sufficiently dilute so that the volume added covers the surface evenly. Note: Coating this recombinant protein at 1-10 µg / well (6 well plate) in T cell specific medium can be used for human T cell differentiation study in vitro.
- Add appropriate amount of diluted material to culture surface.
- Incubate at room temperature for approximately 1 – 2 hours.
- Aspirate remaining material.
- Rinse plates carefully with dH2O– avoid scratching bottom surface of plates.
- Plates are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.
References:
Schraven,B., et al. Association of CD2 and CD45 on human T lymphocytes. Nature 345 (6270), 71-74 (1990)
Baecher-Allan,C.M., et al. CD2 costimulation reveals defective activity by human CD4+CD25(hi) regulatory cells in patients with multiple sclerosis. J. Immunol. 186 (6), 3317-3326 (2011)
Kim,E.O., et al. Homotypic cell to cell cross-talk among human natural killer cells reveals differential and overlapping roles of 2B4 and CD2. J. Biol. Chem. 285 (53), 41755-41764 (2010)
