VitroCol®
Human Collagen Solution, Type I, 3 mg/mL, 20 mL
Catalog #5007-A
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Directions for UseProduct BrochureMSDSDeclaration of Human Source MaterialCertificate of Analysis |
Full Product Description
VitroCol® collagen is the first widely available, naturally produced purified human collagen for research purposes. VitroCol® sets the standard for purity (>99.9% collagen content), functionality and represents the only native-like human collagen offered.
VitroCol® collagen is naturally secreted from human neo-natal fibroblast cells. The human fibroblasts are cultured in optimal conditions allowing the fibroblast to naturally and efficiently secret extracellular matrix. The extracellular matrix is then processed and purified to yield the naturally produced human collagen.
VitroCol® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography.
VitroCol® is supplied at approximately 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. VitroCol® is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0.
VitroCol® is especially ideal for human cell culture systems when coating of surfaces, providing preparations of thin layers of culturing cells, or use as a solid gel. VitroCol® human collagen is provided in user-friendly packaging for use and storage. VitroCol® is sterile filtered and is supplied as a ready to use solution.
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Parameter, Testing, and Method |
VitroCol® Collagen Solution |
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Form |
Solution |
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Package Size |
20 mL |
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Sterilization Method |
Filtration |
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Storage Temperature |
2 to 10°C |
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Expiration Date |
Listed on product label and |
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Concentration |
2.9-3.2 mg/ml |
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Purity |
> 99.9% |
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pH |
Approx. pH 2, 0.01 N HCl |
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Gel Time |
< 90 minutes |
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Fibrillogenesis |
>0.5 Absorbance Units |
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Polyacrylamide Gel Electrophoresis |
> 85% collagen containing within alpha, beta, |
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Sterility |
No growth |
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Endotoxin (LAL) |
< 1.0 EU/ml |
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Cell Attachment Assay |
Pass |
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Source |
Human, Neo-Natal, Fibroblasts |
Preparation and Usage
Note: Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.
- Transfer desired volume of collagen solution from the bottle to a dilution vessel as required. Dilute to desired concentration using sterile 0.01 N HCl solution. A typical working concentration may range from 10 to 100 μg/ml.
- Add appropriate amount of diluted VitroCol® material to the culture surface.
- Incubate at room temperature or 37°C, covered, for 1-2 hours.
- After incubation, aspirate any remaining material.
- Rinse coated surfaces carefully with sterile medium or PBS, avoid scratching surfaces.
- Coated surfaces are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.
3-D Gel Preparation Procedure
- Slowly add 1 part of chilled 10X PBS of 10X culture media to 8 parts of chilled collagen solution with gentle swirling.
- Adjust pH of mixture to 7.2-7.6 using sterile 0.1M NaOH. Monitor pH adjustment carefully (pH meter, phenol red, or pH paper)
- Adjust final volume to a total of 10 parts with sterile water.
- To prevent gelation, maintain temperature of mixture at 2 – 10°C.
- To form gel, warm to 37°C. Allow approximately 90 to 120 minutes for gel formation.
Cell Attachment Bioassay
| Without VitroCol® | With VitroCol® |
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| Human Umbilical Vein Endothelial Cells (HUVEC) 10X | |
 |
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| VERO Epithelial Cells 10X | |
 |
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| Human Dermal Fibroblast Cells 10X | |
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| MDCK Epithelial Cells 10X | |
