PureCol®-S
Collagen Standard Solution, 3 mg/ml, 20 ml
Catalog #5015-A
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Directions for UseMSDSMaterial SourceCertificate of Analysis |
Full Product Description
PureCol® -S collagen is provided as a standard for use in assays where ultra-pure collagen is required. PureCol®-S collagen standard is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography. This product is supplied at approximately a 3 mg/ml concentration. The concentration is confirmed by Biuret protein determination assay. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. PureCol®-S is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0.
PureCol® -S is ideal for using as a collagen standard in controlled testing and assay procedures. PureCol®-S is sterile filtered and is supplied as a ready to use solution.
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Parameter, Testing, and Method |
PureCol®-S Collagen Standard Solution |
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Sterilization Method |
Filtration |
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Form |
Solution |
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Package Size |
20 ml |
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Storage Temperature |
2-10 °C |
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Expiration Date |
Listed on product label and Certificate of Analysis |
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Concentration (Biuret Protein Determination) |
2.9-3.2 mg/mL |
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Purity (collagen concentration) |
> 99.9% |
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pH |
Approx. pH 2, 0.01 N HCl |
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Gel Time |
< 40 minutes |
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Fibrillogenesis |
>0.5 Absorbance Units |
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Polyacrylamide Gel Electrophoresis |
> 85% collagen containing within alpha, beta, and gamma bands < 15% collagen contained with bands traveling faster than alpha |
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Sterility |
No growth |
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Endotoxin (LAL) |
< 1.0 EU/ml |
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Cell Attachment Assay |
Pass |
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Source |
Bovine Hide – Pepsin Extracted |
Preparation and Usage
Note: Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.
- Remove required quantity of collagen from the bottle and dispense into a dilution vessel.
- Dilute PureCol®-S in water to ~50 to 100 µg/ml (~1:30). A 0.01 N HCl solution may also be used.
- Swirl contents gently until material is completely mixed.
- Add appropriate amount of diluted PureCol®-S material to the culture surface ensuring that the entire surface is coated.
- Incubate at room temperature or 37°C, covered, for 1- 2 hours.
- After incubation, aspirate any remaining material.
- Rinse coated surfaces carefully with sterile medium or PBS, avoid scratching surfaces.
- Coated surfaces are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.
3-D Gel Preparation Procedure
- Slowly add 1 part of chilled 10X PBS or 10X culture media to 8 parts of chilled collagen solution with gentle swirling.
- Adjust pH of mixture to 7.2–7.6 using sterile 0.1 M NaOH. Monitor pH adjustment carefully (pH meter, phenol red, or pH paper).
- Adjust final volume to a total of 10 parts with sterile water.
- To prevent gelation, maintain temperature of mixture at 2–10°C.
- To form gel, warm to 37°C. Allow approximately 90 to 120 minutes for gel formation.
